Journal: eLife

Article Title: Continuous muscle, glial, epithelial, neuronal, and hemocyte cell lines for Drosophila research

doi: 10.7554/eLife.85814

Figure Lengend Snippet:

Article Snippet: Antibody , 3E8-3D3 (mouse monoclonal) , Developmental Studies Hybridoma Bank , Cat. # 3E8-3D3, RRID: AB_2721944 , 3E8-3D3 was deposited to the DSHB by Saide, J.D.; mouse monoclonal; IF (1:100).

Techniques: Subcloning, Transfection, Construct, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Software

Journal: Antiviral research

Article Title: Anti-respiratory syncytial virus (RSV) G monoclonal antibodies reduce lung inflammation and viral lung titers when delivered therapeutically in a BALB/c mouse model

doi: 10.1016/j.antiviral.2018.04.014

Figure Lengend Snippet: Linear representation of the 298 aa RSV G protein, with the cytoplasmic domain (CD), the transmembrane domain (TM), the conserved central region (CCR), and CXC3 binding region labeled. The amino acid sequence of residues 162-186 are shown with the four cysteine residues participating in two disulfide bonds. Peptide reactivities of 2B11, 3D3, 3G12, and 131-2G mAbs are shown above the cartoon representation of G.

Article Snippet: On day 3 p.i., at least 4 mice per group were i.p. treated with 5 mg/kg, body weight with anti-RSV G protein mAb 2B11, mAb 3D3, anti-RSV F mAb Palivizumab or control normal mouse immunoglobulin G (IgG), nIg (Trellis Bioscience).

Techniques: Binding Assay, Labeling, Sequencing

Journal: Antiviral research

Article Title: Anti-respiratory syncytial virus (RSV) G monoclonal antibodies reduce lung inflammation and viral lung titers when delivered therapeutically in a BALB/c mouse model

doi: 10.1016/j.antiviral.2018.04.014

Figure Lengend Snippet: RSV infected mice were prophylactically treated with 5 or 1.5 mg/kg isotype control, anti-F (Palivizumab), or anti-G mAbs (2B11 or 3D3) one day prior to inoculation with RSV A2. Lungs were collected at day 5 and used to test for (A) Virus titers (PFU/g of lung tissue) and (B) Level of viral replication as determined by reverse qRT-PCR for M gene expression (relative genome level equivalent PFU/g of lung tissue). BAL were also collected at day 5 pi and used for (C) Mean number of cells (cells per ml) recovered from BAL fluid, (D) amount of IFN-γ in BAL cell-free fluid, (pg/ml) and (E) amount of IL-4 levels in BAL cell-free fluid (pg/ml). Asterisks indicate a significant difference (p < 0.05) between nIg-treated mice and mAbs treated mice as calculated with student’s T test ND, not detectable (levels of virus were below the limit of detection). Results are representative of three independent experiments with three to four mice per time point.

Article Snippet: On day 3 p.i., at least 4 mice per group were i.p. treated with 5 mg/kg, body weight with anti-RSV G protein mAb 2B11, mAb 3D3, anti-RSV F mAb Palivizumab or control normal mouse immunoglobulin G (IgG), nIg (Trellis Bioscience).

Techniques: Infection, Control, Virus, Quantitative RT-PCR, Gene Expression

Journal: Antiviral research

Article Title: Anti-respiratory syncytial virus (RSV) G monoclonal antibodies reduce lung inflammation and viral lung titers when delivered therapeutically in a BALB/c mouse model

doi: 10.1016/j.antiviral.2018.04.014

Figure Lengend Snippet: Enumeration of cell types present in BAL as characterized by flow cytometry staining in mice treated prophylactically with isotype control, anti-F, and anti-G mAbs prior to inoculation with RSV A2.

Article Snippet: On day 3 p.i., at least 4 mice per group were i.p. treated with 5 mg/kg, body weight with anti-RSV G protein mAb 2B11, mAb 3D3, anti-RSV F mAb Palivizumab or control normal mouse immunoglobulin G (IgG), nIg (Trellis Bioscience).

Techniques: Flow Cytometry, Staining, Control

Journal: Antiviral research

Article Title: Anti-respiratory syncytial virus (RSV) G monoclonal antibodies reduce lung inflammation and viral lung titers when delivered therapeutically in a BALB/c mouse model

doi: 10.1016/j.antiviral.2018.04.014

Figure Lengend Snippet: RSV-infected mice were treated with 5 mg/kg with anti-G protein mAbs 2B11, 3D3, anti-F mAb Palivizumab and isotype control on Day 3 post-inoculation. Lungs were collected on days 3, 5, 7, 10 and day 14 pi and used for (A) Virus titers (PFU/g of lung tissue) BAL were collected on days 3,5,7,10 and day 14 pi. and used for (B) Mean number of cells (cells per ml) recovered from BAL fluid, (C) amount of IFN-γ in BAL cell free fluid (pg/ml), and (D) amount ofIL-4 levels in BAL cell-free fluid (pg/ml). Asterisks indicate a significant difference (p < 0.05) between nlg-treated mice and mAbs treated mice as calculated by Student’s T test. The arrows indicate the time of treatment. ND, not detectable (levels of virus were below the limit of detection). Results are representative of three independent experiments with four mice per time point.

Article Snippet: On day 3 p.i., at least 4 mice per group were i.p. treated with 5 mg/kg, body weight with anti-RSV G protein mAb 2B11, mAb 3D3, anti-RSV F mAb Palivizumab or control normal mouse immunoglobulin G (IgG), nIg (Trellis Bioscience).

Techniques: Infection, Control, Virus

Journal: Antiviral research

Article Title: Anti-respiratory syncytial virus (RSV) G monoclonal antibodies reduce lung inflammation and viral lung titers when delivered therapeutically in a BALB/c mouse model

doi: 10.1016/j.antiviral.2018.04.014

Figure Lengend Snippet: BAL were collected on days 3, 5, 7, 10, and 14 days post RSV A2 inoculation in mice treated with 5 mg/kg isotype control, anti-F protein (Palivizumab), human anti-G protein mAbs (2B11 or 3D3), or mouse anti-G 131-2G mAbs at 3 days post-inoculation. BAL cells were stained and used for flow cytometry characterization and enumeration with antibodies against (A) B220+ cells (B) DX5+ cells, (C) CD3+ cells, and (D) CD8+ cells. The data are expressed as the number of BAL/lung ( ± SEM). A representative experiment from three independent experiments is shown. Asterisks indicate a significant difference (P < 0.05) between isotype control-treated and antibody-treated mice as calculated with a Student’s T test. The arrows indicate the time of treatment.

Article Snippet: On day 3 p.i., at least 4 mice per group were i.p. treated with 5 mg/kg, body weight with anti-RSV G protein mAb 2B11, mAb 3D3, anti-RSV F mAb Palivizumab or control normal mouse immunoglobulin G (IgG), nIg (Trellis Bioscience).

Techniques: Control, Staining, Flow Cytometry

Journal: Antiviral research

Article Title: Anti-respiratory syncytial virus (RSV) G monoclonal antibodies reduce lung inflammation and viral lung titers when delivered therapeutically in a BALB/c mouse model

doi: 10.1016/j.antiviral.2018.04.014

Figure Lengend Snippet: Lung histopathology score (LHS) at 5 days post infection.

Article Snippet: On day 3 p.i., at least 4 mice per group were i.p. treated with 5 mg/kg, body weight with anti-RSV G protein mAb 2B11, mAb 3D3, anti-RSV F mAb Palivizumab or control normal mouse immunoglobulin G (IgG), nIg (Trellis Bioscience).

Techniques: Histopathology, Infection

Journal: Antiviral research

Article Title: Anti-respiratory syncytial virus (RSV) G monoclonal antibodies reduce lung inflammation and viral lung titers when delivered therapeutically in a BALB/c mouse model

doi: 10.1016/j.antiviral.2018.04.014

Figure Lengend Snippet: Lung histopathology was examined by H&E staining in (A) mock-infected mice or mice infected with RSV A2 and then treated with (B) isotype control antibody, (C) anti-G mAb 3D3, (D) anti-G mAb 2B11 (E) anti-F mAb Palivizumab, or (F) anti-G mAb 131-2G. The arrows indicate areas of inflammatory infiltrates. There are lymphocytes and plasma cells (solid black arrow) around a venule (V). 200× original magnification, scale bar = 50 μm.

Article Snippet: On day 3 p.i., at least 4 mice per group were i.p. treated with 5 mg/kg, body weight with anti-RSV G protein mAb 2B11, mAb 3D3, anti-RSV F mAb Palivizumab or control normal mouse immunoglobulin G (IgG), nIg (Trellis Bioscience).

Techniques: Histopathology, Staining, Infection, Control, Clinical Proteomics